Double Beam Spectrophotometer

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Double Beam Spectrophotometer Specification

Feature
High precision measurements, rapid scanning, auto zero function

Power Source
220V AC main supply

Voltage
220 V

Accuracy
0.003 Abs at 1.0 Abs

Core Components
Optical system, monochromator, detector, light source

Measurement Range
190-1100 nm

Capacity
2 ml minimum sample volume

Automation Grade
Semi-Automatic

Model No
SPUV-1200DB

Temperature Range
Ambient to 45C

Frequency
50/60 Hz

Equipment Materials
High-quality ABS and metal housing

Type
Double Beam Spectrophotometer

Usage
UV/VIS analysis, quantitative and qualitative assay

Display Type
Large LCD Display

Dimension (L*W*H)
480 x 380 x 160 mm

Weight
Approx. 15 kg

Spectral Bandwidth
2 nm

PC Interface
USB / RS-232

Stray Light
0.05%T @ 220 nm, 340 nm

Software
Compatible with Windows OS; data export and graphing features

Photometric Range
-0.3 - 3.0 A

Sample Volume
0.5 - 3.5 ml (depends on cuvette size)

Detector Type
Silicon Photodiode Array

Stability
0.001 A/h @ 500 nm

Application
Research, Academic, Pharmaceutical, Industrial testing

Wavelength Reproducibility
0.2 nm

Noise Level
0.001 A

Resolution
0.1 nm

Wavelength Accuracy
0.3 nm

Cuvette Holder Type
4-position automatic changer (optional upgrade)

Lamp Life
2000 hr (Deuterium), 3000 hr (Tungsten Halogen)

About Double Beam Spectrophotometer

Double Beam Spectrophotometer offered Comes with wavelength range from 190-1100 nm, spectral bandwidth of 1 nm and working mode of T,A,C,E. Also featuring double beam based optical system, it can deliver slow, medium and fast scanning speeds as per he requirements. Further for more efficient performance, the system is capable of delivering photometric accuracy of + 0.3% T (0-100% T) & +0.002 A (0-0.1 A), photometric reproducibility of < 0.15 % T, stability of < 0.001A/h (at 500 nm, after warming up). Some of the other standard features of the system include 6 inches high light blue LCD display support, options of glass cells, quartz cells, fuse, instrument cover, software CD and RS 232 cable among others.

Technical Specification:-

Wavelength Range	190-1100 nm
Spectral Bandwidth	1 nm
Working Mode	T,A,C,E
Optical System	Double beam
Scanning Speed	Fast / Medium / Slow
Photometric Range	-0.3 - 3.5 A, 0 to 220 %T
Wavelength Accuracy	+0.3 nm
Wavelength Reproducibility	< 0.10 nm
Photometric Accuracy	+ 0.3% T (0-100% T) +0.002 A (0-0.1 A)
Photometric Reproducibility	< 0.15 % T
Stray Light	< 0.1% T (Nal. 220nm, NaNO2 340 nm)
Baseline Flatness	+ 0.002 A
Stability	< 0.001A/h (at 500 nm, after warming up)
Noise	+0.001A (at 500 nm, after warming up)
Detector	Dual Silicon Photodiode
Display	6 inches high light blue LCD
Power	AC:220V + 10%, 50 Hz.
Dimensions	630 x 470 x 210 mm
Weight	26 Kg
Glass Cells	16 No.
Quartz Cells	4 Nos.
Fuse	3 Nos.
Instrument Cover	1 No.
Software CD	1 No.
RS 232 Cable	1 No.
Operation Manual	1 No.

High Accuracy and Excellent Stability

Benefit from superior accuracy (0.003 Abs at 1.0 Abs) and stability ( 0.001 A/h @ 500 nm), ensuring reliable and reproducible results for every measurement. The optical system and silicon photodiode array detector minimize noise ( 0.001 A), making the SPUV-1200DB ideal for sensitive assays and demanding research environments.

User-Friendly Features and Flexible Operation

Designed with semi-automatic operation and a large LCD display, this spectrophotometer simplifies workflows in academic, pharmaceutical, and industrial labs. Its Windows-compatible software, with robust data export and graphing capabilities, supports efficient analysis, while optional automation and a wide sample volume range accommodate diverse application needs.

Durable Construction for Rigorous Applications

Constructed with high-quality ABS and metal housing, the SPUV-1200DB withstands intensive laboratory use. It operates within an ambient to 45C temperature range and features cost-effective lamps (2000 h deuterium, 3000 h tungsten halogen), ensuring long-term, dependable service for distributors, manufacturers, and end-users alike.

FAQs of Double Beam Spectrophotometer:

Q: How does the double beam feature benefit UV/VIS analysis in the SPUV-1200DB?

A: The double beam design allows simultaneous measurement of sample and reference beams, reducing baseline drift and compensating for fluctuations. This improves accuracy and stability, essential for quantitative and qualitative UV/VIS assays in research and industry.

Q: What types of samples and volumes can the spectrophotometer analyze?

A: The SPUV-1200DB accommodates sample volumes from 0.5 to 3.5 ml, depending on cuvette size, with a minimum of 2 ml recommended. It is suitable for a wide range of solutions typically encountered in chemical, pharmaceutical, and academic laboratories.

Q: When should the lamps be replaced, and what is their expected lifespan?

A: The deuterium lamp provides up to 2000 hours, while the tungsten halogen lamp offers around 3000 hours of use. Replacement is recommended near the end of their rated lifespans or when light intensity decreases, ensuring consistent measurement performance.

Q: Where can the SPUV-1200DB spectrophotometer be used effectively?

A: This model performs excellently in research institutes, academic labs, pharmaceutical analysis, and industrial testing facilities across India and globally, thanks to its robust construction and precise analytical capabilities.

Q: What is the process for exporting data and creating graphs with this spectrophotometer?

A: Using the included Windows-compatible software, users can export measurement data via USB or RS-232 and leverage built-in graphing features for immediate visualization or reporting, streamlining the analysis workflow.

Q: How does the automatic cuvette changer enhance laboratory efficiency?

A: The optional 4-position automatic cuvette changer enables rapid, sequential measurement of multiple samples, reducing manual intervention and improving throughput, especially during high-volume testing or batch analysis.

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